1533
1533 .
MARINIFILUM MEDIUM (IRD MEDIUM)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1533 #
ml
Equipment needed: Anaerobic workbench
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| NaCl | 25.0 | g | 25 | 427.789 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Cysteine HCl | 0.5 | g | 0.5 | 4.127 | |
|
Yeast extract
(Oxoid) |
1.0 | g | 1 | - | |
| Trace element solution SL-7 | 1.0 | mL | - | - | |
|
Resazurin
(0.1%) |
1.0 | mL | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Add after autoclave | |||||
|
MgCl2 x 6 H2O
(150.0 g/l) |
20.0 | ml/l | 3 | 14.756 | |
|
NaHCO3
(10%) |
20.0 | ml/l | 2 | 23.808 | |
|
Na2S x 9 H2O
(2%) |
20.0 | ml/l | 0.4 | 1.665 | |
|
Glucose
(1M) |
20.0 | ml/l | 3.603 | 20 | |
| 2 pH 7.0 | |||||
| 3 Prepare the medium without NaHCO3, Na2S x 9 H2O, MgCl2 x 6 H2O and glucose. Boil the medium in a water bath and cool under a stream of N2/CO2 (80/20). Add the NaHCO3 to the cooled medium (the pH should be 7.0) and dispense the medium into Hungate tubes or serum bottles under a stream of N2/CO2 (80/20). Seal the tubes under N2/CO2 (80/20) and autoclave. To the cooled medium at the appropriate amounts of Na2S x 9 H2O, MgCl2 x 6 H2O and glucose from sterile, anaerobic stock solutions. | |||||
Solution occurs in 1
other media (first defined in Medium 941).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Distilled water | 1000.0 | ml | - | - |
|
HCl
(25%) |
6.5 | ml | 1.625 | 44.569 |
| FeCl2 x 7 H2O | 1.5 | g | 1.5 | 11.834 |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 |
| H3BO3 | 62.0 | mg | 0.062 | 1.003 |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 25 | 427.789 |
| Glucose | 3.603 | 20 |
| MgCl2 x 6 H2O | 3 | 14.756 |
| NaHCO3 | 2 | 23.808 |
| NH4Cl | 1 | 18.695 |
| Show more | ||
