DSMZ 1533 . MARINIFILUM MEDIUM (IRD MEDIUM)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Balneicella halophila DSM 28579

This strain uses the standard medium without modifications.

Equipment needed: Anaerobic workbench Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.3 2.205
K2HPO4 0.3 g 0.3 1.722
NH4Cl 1.0 g 1 18.695
NaCl 25.0 g 25 427.789
KCl 0.1 g 0.1 1.341
CaCl2 x 2 H2O 0.1 g 0.1 0.68
Cysteine HCl 0.5 g 0.5 4.127
Yeast extract
(Oxoid)
1.0 g 1 -
Trace element solution SL-7 1.0 mL - -
Resazurin
(0.1%)
1.0 mL 1.0e-3 0.004
Distilled water 1000.0 ml - -
1 Add after autoclave
MgCl2 x 6 H2O
(150.0 g/l)
20.0 ml/l 3 14.756
NaHCO3
(10%)
20.0 ml/l 2 23.808
Na2S x 9 H2O
(2%)
20.0 ml/l 0.4 1.665
Glucose
(1M)
20.0 ml/l 3.603 20
2 pH 7.0
3 Prepare the medium without NaHCO3, Na2S x 9 H2O, MgCl2 x 6 H2O and glucose. Boil the medium in a water bath and cool under a stream of N2/CO2 (80/20). Add the NaHCO3 to the cooled medium (the pH should be 7.0) and dispense the medium into Hungate tubes or serum bottles under a stream of N2/CO2 (80/20). Seal the tubes under N2/CO2 (80/20) and autoclave. To the cooled medium at the appropriate amounts of Na2S x 9 H2O, MgCl2 x 6 H2O and glucose from sterile, anaerobic stock solutions.
Solution occurs in 1 other media (first defined in Medium 941).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.0 ml - -
HCl
(25%)
6.5 ml 1.625 44.569
FeCl2 x 7 H2O 1.5 g 1.5 11.834
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Balneicella halophila DSM 28579

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
Glucose 3.603 20
MgCl2 x 6 H2O 3 14.756
NaHCO3 2 23.808
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain