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DSMZ

DSMZ 1534 . MARINIFILUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1534 #

ml
Equipment needed: Autoclave Hungate tubes Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 13.00 g 13 222.45
MgCl2 x 6 H2O 2.80 g 2.8 13.773
KCl 0.36 g 0.36 4.829
NH4Cl 0.25 g 0.25 4.674
KH2PO4 0.20 g 0.2 1.47
CaCl2 x 2 H2O 0.70 g 0.7 4.761
Na2SO4 1.42 g 1.42 9.997
Glucose 2.00 g 2 11.101
Resazurin
(0.1%) 		1.00 ml 1.0e-3 0.004
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NaHCO3 2.52 g 2.52 29.998
Na2S x 9 H2O 0.50 g 0.5 2.082
Vitamin solution V7 1.00 ml - -
Distilled water 1000.00 ml - -
1 pH 7.0- 7.2
2 Prepare the medium without NaHCO3, Na2S x 9 H2O, vitamin solution, Selenite-tungstate solution and glucose. Boil the medium in a water bath and cool under a stream of N2/CO2 (80/20). Add the NaHCO3 to the cooled medium (the pH should be 7.0-7.2) and dispense the medium into Hungate tubes or serum bottles under a stream of N2/CO2 (80/20). Seal the tubes under N2/CO2 (80/20) and autoclave. To the cooled medium at the appropriate amounts of Na2S x 9 H2O, vitamin solution, Selenite-tungstate solution and glucose from sterile, anaerobic stock solutions.

Selenite-tungstate solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
NaOH 0.5 g 0.5 12.501
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution V7 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
p-Aminobenzoic acid 50 mg 0.05 0.365
Vitamin B12 50 mg 0.05 0.037
Thiamine HCl 50 mg 0.05 0.148
Nicotinic acid 100 mg 0.1 0.812
Calcium D-(+)-pantothenate 25 mg 0.025 -
Pyridoxamine Dihydrochloride 250 mg 0.25 1.037
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Anaerobic workbench, Autoclave, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 13 222.45
MgCl2 x 6 H2O 2.8 13.773
NaHCO3 2.52 29.998
Glucose 2 11.101
Na2SO4 1.42 9.997
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Cultivation metadata from 2 strains BacDive

phyla 1 4 strains
temperature mesophilic 3 strains
temperature psychrophilic 2 strains