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1539. THERMOTOGA PROFUNDA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.05 g 0.049 0.281
2 0.09 g 0.088 0.648
3 0.25 g 0.245 4.582
4 0.25 g 0.245 0.994
5 0.15 g 0.147 1
6 1.00 g 0.98 -
7 Trace element solution 10.00 ml - -
8
(0.1% w/v)
0.50 ml 4.9e-4 0.002
9 2.00 g 1.961 18.5
10 2.00 g 1.961 10.884
11 Vitamin solution 10.00 ml - -
12 0.30 g 0.294 1.675
13 0.30 g 0.294 1.225
14 1000.00 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients except carbonate, glucose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.5, if necessary.
Solution occurs in 14 other media (first defined in medium 318 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(NTA)
12.80 g 12.8 66.967
2 1.00 g 1 5.03
3 0.10 g 0.1 0.505
4 0.03 g 0.03 0.126
5 0.10 g 0.1 0.68
6 0.10 g 0.1 0.734
7 0.02 g 0.02 0.149
8 0.01 g 0.01 0.162
9 0.03 g 0.03 0.124
10 0.10 g 0.1 0.421
11 1.00 g 1 17.111
12 0.03 g 0.03 0.114
13 0.04 g 0.04 0.121
14 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2CO3 1.961 18.5
D-Glucose 1.961 10.884
Yeast extract 0.98 -
L-Cysteine HCl x H2O 0.294 1.675
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 2 strains
temperature thermophilic 3 strains

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