1556
1556 .
BM7 MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1556 #
ml
Equipment needed: Autoclave
| 1 Cultivation of Liberibacter sp. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ACES | 10.00 | g | 10 | 54.885 | |
| Na2 alpha-ketoglutarate | 2.00 | g | 2 | 13.881 | |
| KOH | 3.75 | g | 3.75 | 66.838 | |
| Distilled water | 550.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 2 adjust pH to 6.9, autoclave for 15 minutes and allow to cool. | |||||
| 3 Add aseptically | |||||
| Fetal bovine serum | 150.00 | ml | - | - | |
|
TMN-FH Medium
(Sigma-Aldrich, T3285) |
300.00 | ml | - | - | |
| 4 For preparation of agar plates add 15 g agar per liter of medium. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| ACES | 10 | 54.885 |
| KOH | 3.75 | 66.838 |
| Na2 alpha-ketoglutarate | 2 | 13.881 |
| Distilled water | - | - |
| Show more | ||
