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1560. PLANCTOMYCES MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Bacto)
0.25 g 0.244 -
2
(Bacto)
0.25 g 0.244 -
3 2.38 g 2.324 -
4 Mineral salts solution 20.00 ml - -
5 Trace element solution 1.00 ml - -
6 Artifical sea water 250.00 ml - -
7
(Bacto)
15.00 g 14.648 -
8 730.00 ml - -
9
(10% stock solution)
8.00 ml - -
10
(2.5%, sterile-filtered)
10.00 ml - -
11 Vitamin solution (double conc.) 5.00 ml - -
Instructions:
  1. Sterilize 20 min./ 121°C. After cooling to 60°C add: #9-11
  2. Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
Solution occurs in 2 other media (first defined in medium 1560 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 46.940 g 46.94 803.183
2 7.840 g 7.84 55.195
3 21.280 g 21.28 104.671
4 2.860 g 2.86 25.77
5 0.380 g 0.38 4.523
6 1.380 g 1.38 18.511
7 0.192 g 0.192 1.613
8 0.052 g 0.052 0.841
9 0.080 g 0.08 0.505
10 0.006 g 0.006 0.143
11 1000.000 ml - -
Solution occurs in 4 other media (first defined in medium 1560 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(NTA)
10.00 g 10 52.318
2 29.70 g 29.7 120.499
3 3.34 g 3.34 22.719
4 12.67 mg 0.013 -
5 99.00 mg 0.099 0.356
6 "Metals 44" 50.00 ml - -
7 950.00 ml 948.297 52638.4
Instructions:
  1. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 37 other media (first defined in medium 1137 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 250.0 mg 0.25 0.744
2 1095.0 mg 1.095 3.808
3 500.0 mg 0.5 1.798
4 154.0 mg 0.154 0.911
5 39.2 mg 0.039 0.157
6 24.8 mg 0.025 0.085
7 17.7 mg 0.018 0.046
8 1000.0 ml 998.207 55408.8
9 , required for dissolving EDTA x - -
Instructions:
  1. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
Solution occurs in 4 other media (first defined in medium 1560 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 5.835
2 0.50 g 0.5 2.958
3 0.10 g 0.1 0.36
4 0.10 g 0.1 0.344
5 0.10 g 0.1 0.734
6 0.05 g 0.05 0.21
7 0.05 g 0.05 0.388
8 0.01 g 0.01 0.04
9 0.01 g 0.01 0.021
10 0.01 g 0.01 0.162
11 0.01 g 0.01 0.041
12 0.01 g 0.01 0.03
13 1000.00 ml - -
Solution occurs in 4 other media (first defined in medium 1560 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.0 mg 0.004 0.016
2 4.0 mg 0.004 0.009
3 20.0 mg 0.02 0.097
4 10.0 mg 0.01 0.027
5 10.0 mg 0.01 0.027
6 10.0 mg 0.01 0.082
7 10.0 mg 0.01 0.021
8 0.2 mg 2.0e-4 1.3e-4
9 10.0 mg 0.01 0.073
10 1000.0 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8

Associated strains

All strains for this medium: 11
Crateriforma conspicua DSM 100706

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 19.496 1082.2
Agar 14.648 -
NaCl 11.46 196.09
MgCl2 x 6 H2O 5.195 25.554
HEPES 2.324 -
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Cultivation metadata from 11 strains BacDive

temperature mesophilic 2 strains

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