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DSMZ

DSMZ 1560a . PLANCTOMYCES MEDIUM WITH DOUBLE CONCENTRATED ARTIFICAL SEA WATER

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Culture medium recipe

Vol.
×

Main sol. 1560a #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone
(Bacto) 		0.25 g 0.25 -
Yeast extract
(Bacto) 		0.25 g 0.25 -
HEPES 2.38 g 2.38 9.987
Mineral salts solution 20.00 ml - -
Trace element solution 1.00 ml - -
Artifical sea water 250.00 ml - -
Agar
(Bacto) 		15.00 g 15 44.599
Double distilled water 730.00 ml - -
1 Sterilize 20 min./121°C. After cooling to 60°C add:
N-Acetylglucosamine
(20g/L stock solution) 		40.00 ml - -
Glucose
(2.5%, sterile-filtered) 		10.00 ml 0.25 1.388
Vitamin solution (double conc.) 5.00 ml - -
2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).

Artifical sea water #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 93.880 g 93.88 1606.43
Na2SO4 15.680 g 15.68 110.391
MgCl2 x 6 H2O 42.560 g 42.56 209.343
CaCl2 5.720 g 5.72 51.541
NaHCO3 0.760 g 0.76 9.047
KCl 2.760 g 2.76 37.022
KBr 0.380 g 0.38 3.193
H3BO3 0.100 g 0.1 1.617
SrCl2 0.160 g 0.16 1.009
NaF 0.012 g 0.012 0.286
Double distilled water 1000.000 ml - -

Mineral salts solution #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.00 g 10 52.318
MgSO4 x 7 H2O 29.70 g 29.7 120.499
CaCl2 x 2 H2O 3.34 g 3.34 22.719
Na2MoO7O4 x 2 H2O 12.67 mg 0.013 -
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
"Metals 44" 50.00 ml - -
Distilled water 950.00 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-Nitrilotriacetat 1.50 g 1.5 -
MnSO4 x H2O 0.50 g 0.5 2.958
Fe SO4 x 7 H2O 0.10 g 0.1 0.36
Co(NO3)2 x 6 H2O 0.10 g 0.1 0.344
ZnCl2 0.10 g 0.1 0.734
NiCl2 x 6 H2O 0.05 g 0.05 0.386
H2SeO3 0.05 g 0.05 0.388
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.01 g 0.01 0.021
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Na2WO4 x 2 H2O 0.01 g 0.01 0.03
Double distilled water 1000.00 ml - -

Vitamin solution (double conc.) #

1000
Solution occurs in 4 other media (first defined in Medium 1560).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Double distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.5
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 23.47 401.608
Agar 15 44.599
MgCl2 x 6 H2O 10.64 52.336
Na2SO4 3.92 27.598
HEPES 2.38 9.987
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