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DSMZ

DSMZ 1561 . Medium for broad range phototrophic bacteria

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1561 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.30 g 0.3 -
NH4Cl 0.50 g 0.5 9.348
KH2PO4 0.50 g 0.5 3.674
MgCl2 x 6 H2O 0.30 g 0.3 1.476
KCl 0.50 g 0.5 6.707
Na2S2O3 0.10 g 0.1 0.233
NaCl 0.50 g 0.5 8.556
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Sodium acetate 0.50 g 0.5 6.095
Sodium malate 0.50 g 0.5 2.808
HEPES 3.00 g 3 12.589
Trace element solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5.
2 Autoclave at 121°C for 15 min. Before inoculation add 1.0 ml/ l 5% sulfide solution pH 7.5 and 1.0 ml/l vitamin solution.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium 1375).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
HEPES 3 12.589
NH4Cl 0.5 9.348
KCl 0.5 6.707
Sodium acetate 0.5 6.095
KH2PO4 0.5 3.674
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
nutrition chemoheterotroph 1 strain
nutrition photoheterotroph 1 strain
oxygen aerobe 1 strain
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