DSMZ 1565 . ALKALIPHILUS TRANSVAALENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
MJ(-N) synthetic seawater 100.00 ml - -
Yeast extract
(BD Bacto)
2.00 g 2 -
Tryptone
(BD Bacto)
2.00 g 2 28.137
Na2-tartrate x 2 H2O 1.00 g 1 -
Na3-citrate 1.00 g 1 3.875
NaNO3 0.25 g 0.25 2.941
(NH4)2SO4 0.25 g 0.25 1.892
Fe(III) citrate 0.20 g 0.2 0.817
Na2SO4 1.00 g 1 7.04
Na2CO3 1.25 g 1.25 11.794
NaHCO3 2.50 g 2.5 29.759
Na2S2O3 x 5 H2O 2.00 g 2 8.058
Distilled water 900.00 ml - -
1 Dissolve ingredients except carbonate, bicarbonate and thiosulfate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and bicarbonate, adjust pH to 11 with KOH, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate to the autoclaved medium from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 9.8 - 10.0.
Solution occurs in 12 other media (first defined in Medium J268).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.703 508.264
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.14 g 0.139 0.943
MgSO4 x 7 H2O 3.40 g 3.366 13.658
MgCl2 x 6 H2O 4.18 g 4.139 20.357
KCl 0.33 g 0.327 4.383
NiCl2 x 6 H2O 0.50 mg 5.0e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 5.0e-4 0.002
Fe(NH4)2(SO4)2 x 6 H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 11.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.8 - 10.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.5 29.759
Tryptone 2 28.137
Na2S2O3 x 5 H2O 2 8.058
Yeast extract 2 -
Na2CO3 1.25 11.794
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain