1565 .
ALKALIPHILUS TRANSVAALENSIS MEDIUM
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Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
9.8 - 10.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MJ(-N) synthetic seawater | 100.00 | ml | ||
|
|
Yeast extract
(BD Bacto) |
2.00 | g | ||
|
|
Tryptone
(BD Bacto) |
2.00 | g | ||
|
|
Na2-tartrate x 2 H2O | 1.00 | g | ||
|
|
Na3-citrate | 1.00 | g | ||
|
|
NaNO3 | 0.25 | g | ||
|
|
(NH4)2SO4 | 0.25 | g | ||
|
|
Ferric citrate monohydrate | 0.20 | g | ||
|
|
Na2SO4 | 1.00 | g | ||
|
|
Na2CO3 | 1.25 | g | ||
|
|
NaHCO3 | 2.50 | g | ||
|
|
Na2S2O3 x 5 H2O | 2.00 | g | ||
|
|
Distilled water | 900.00 | ml | ||
| 1 Dissolve ingredients except carbonate, bicarbonate, and thiosulfate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and bicarbonate, adjust pH to 10 with KOH, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate to the autoclaved medium from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust the pH of the complete medium to 9.8 - 10.0. | |||||
MJ(-N) synthetic seawater #
1010
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 30.00 | g |
|
|
K2HPO4 | 0.14 | g |
|
|
CaCl2 x 2 H2O | 0.14 | g |
|
|
MgSO4 x 7 H2O | 3.40 | g |
|
|
MgCl2 x 6 H2O | 4.18 | g |
|
|
KCl | 0.33 | g |
|
|
NiCl2 x 6 H2O | 0.50 | mg |
|
|
Na2SeO3 x 5 H2O | 0.50 | mg |
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g |
|
|
Modified Wolin's mineral solution | 10.00 | ml |
|
|
Distilled water | 1000.00 | ml |
Modified Wolin's mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.18 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.18 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 x 12 H2O | 0.02 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.30 | mg | ||
|
|
Na2WO4 x 2 H2O | 0.40 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||