156
156 .
ANAEROTIGNUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Anaerotignum propionicum DSM 1682
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
35 °C
Main sol. 156 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| L-Alanine | 3.0 | g | 3 | 33.674 | |
| Peptone | 3.0 | g | 3 | - | |
| Yeast extract | 4.0 | g | 4 | - | |
| MgSO4 x 7 H2O | 0.1 | g | 0.1 | 0.406 | |
| FeSO4 x 7 H2O | 18.0 | mg | 0.018 | 0.065 | |
|
Potassium phosphate buffer
(1 M, pH 7.1) |
5.0 | ml | - | 5 | |
|
CaSO4
(saturated aq. solution) |
2.5 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 1.0 | g | 1 | 11.904 | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.3 | 1.708 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate and cysteine), adjust pH to 7.0, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and cysteine, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or seruim vials and autoclave. Adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Anaerotignum propionicum DSM 1682
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 4 | - |
| L-Alanine | 3 | 33.674 |
| Peptone | 3 | - |
| NaHCO3 | 1 | 11.904 |
| L-Cysteine HCl x H2O | 0.3 | 1.708 |
| Show more | ||
