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1571. MICROBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.41 g 0.41 3.013
2 0.53 g 0.53 2.978
3 0.30 g 0.3 5.608
4 0.30 g 0.3 5.133
5 0.10 g 0.1 0.492
6 0.01 g 0.01 0.068
7 2.00 g 2 -
8 0.36 g 0.36 1.499
9 0.50 g 0.5 2.182
10 Trace element solution SL-10 1.00 ml - -
11 0.50 g 0.5 4.127
12 Vitamin solution 0.50 ml - -
13 Selenite-tungstate solution 0.50 ml - -
14 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve in 1 liter, boil under N2, add 0.5 g of L-cysteine and adjust pH to 6.4 (should be pH 5.5 after autoclaving). Disperse in tubes and bottles (leave 50% of container volume as gas phase). Change to gas mixture N2/CO2; 80/20%) and autoclave for 20 minutes at 121°C.
  2. To 100 ml sterile medium add aseptically: #12-13
Solution occurs in 32 other media (first defined in medium 461 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%; 7.7 M)
10.0 ml 3.17 86.943
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 12 other media (first defined in medium 1668 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.013
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Instructions:
  1. Filter sterilize and make anoxic with N2.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1000.19 55519.1
Yeast extract 2 -
Na2HPO4 x 2 H2O 0.53 2.978
L-Cysteine 0.5 4.127
Resazurin 0.5 2.182
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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