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DSMZ

DSMZ 1583 . MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Nitrosomonas europaea DSM 28437

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 1583 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 535 mg 0.535 10.002
KH2PO4 54 mg 0.054 0.397
KCl 74 mg 0.074 0.993
MgSO4 x 7 H2O 49 mg 0.049 0.199
CaCl2 x 2 H2O 147 mg 0.147 1
NaCl 584 mg 0.584 9.993
Trace element solution 1 ml - -
Cresol red solution 0.5 g/L 2 ml - -
Distilled water 1000 ml - -
1 Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile 10% NaHCO3 (medium is turning from yellow to pink).
2 Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES may be added to keep pH neutral for a longer time.
3 Aeration must be adjusted according to the density and activity of the culture. At low cell concentrations (which is the case most of the time) cells are sensitive to strong aeration (don't shake the cultures).

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 975 ml - -
HCl , 1M 25 ml - -
MnSO4 x 4 H2O 45 mg 0.045 0.202
H3BO3 49 mg 0.049 0.792
ZnSO4 x 7 H2O 43 mg 0.043 0.15
(NH4)6Mo7O24 x 4 H2O 37 mg 0.037 -
FeSO4 x 7 H2O 973 mg 0.973 3.5
CuSO4 x 5 H2O 25 mg 0.025 0.1

Cresol red solution 0.5 g/L #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cresol red 50 mg 0.5 1.307
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.8
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 5
Nitrosomonas europaea DSM 28437

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.584 9.993
NH4Cl 0.535 10.002
CaCl2 x 2 H2O 0.147 1
KCl 0.074 0.993
KH2PO4 0.054 0.397
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Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
temperature mesophilic 5 strains