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DSMZ

DSMZ 1583 . MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL)

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Equipment needed: Autoclave
Final pH: 7.8
Select a strain (optional):

ml
Compound Amount Unit
NH4Cl 535 mg
KH2PO4 54 mg
KCl 74 mg
MgSO4 x 7 H2O 49 mg
CaCl2 x 2 H2O 147 mg
NaCl 584 mg
Trace element solution 1 ml
Cresol red solution 0.5 g/L 2 ml
Distilled water 1000 ml
1 Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile 10% NaHCO3 (medium is turning from yellow to pink).
2 Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES may be added to keep pH neutral for a longer time.
3 Aeration must be adjusted according to the density and activity of the culture. At low cell concentrations (which is the case most of the time) cells are sensitive to strong aeration (don't shake the cultures).

Trace element solution #

1000
Compound Amount Unit
Distilled water 975 ml
HCl , 1M 25 ml
MnSO4 x 4 H2O 45 mg
H3BO3 49 mg
ZnSO4 x 7 H2O 43 mg
(NH4)6Mo7O24 x 4 H2O 37 mg
FeSO4 x 7 H2O 973 mg
CuSO4 x 5 H2O 25 mg

Cresol red solution 0.5 g/L #

100
Compound Amount Unit
Cresol red 50 mg
Distilled water 100 ml