1585
1585 .
SULFURIFERULA MULTIVORANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1585 #
ml
Equipment needed: Serum bottle
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 12 H2O | 3.00 | g | 3 | 21.133 | |
| KH2PO4 | 1.80 | g | 1.8 | 13.227 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.757 | |
| CaCl2 x 2 H2O | 0.04 | g | 0.04 | 0.272 | |
| FeCl3 x 6 H2O | 0.03 | g | 0.03 | 0.111 | |
| MnSO4 x 5 H2O | 0.03 | g | 0.03 | 0.124 | |
| Na2S2O3 x 5 H2O | 10.00 | g | 10 | 40.292 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except thiosulfate and adjust pH of medium to 6.5 -7.0. Dispense medium under air atmosphere into serum vials suitable for anaerobic cultivation (e.g., 20 ml medium in 100 ml bottles) and close with butyl rubber septa. After autoclaving complete medium with thiosulfate added from a stock solution sterilized by filtration and add 3% (v/v) sterile CO2 to the gas phase. | |||||
Metadata
| Last modified: | 11.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.5 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Serum bottle |
| Edit in Medium builder | |
Gas composition
- 97 % Air
- 3 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2S2O3 x 5 H2O | 10 | 40.292 |
| Na2HPO4 x 12 H2O | 3 | 21.133 |
| KH2PO4 | 1.8 | 13.227 |
| (NH4)2SO4 | 0.1 | 0.757 |
| MgSO4 x 7 H2O | 0.1 | 0.406 |
| Show more | ||
