This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(Difco) |
4 | g | 4 | - | |
Malt extract
(Difco) |
10 | g | 10 | - | |
Dextrose
(Difco) |
4 | g | 4 | 20.185 | |
Trace element solution SL-10 | 1 | ml | - | - | |
Agar | 20 | g | 20 | 59.465 | |
Distilled water | 1000 | ml | - | - | |
1 pH 7.2 | |||||
2 Sterilize 20 min/121°C. After cooling to 60°C add to the medium: | |||||
Vitamin solution | 5 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(25%; 7.7 M) |
10.0 | ml | 2.5 | 68.568 |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 |
H3BO3 | 6.0 | mg | 0.006 | 0.097 |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
Distilled water | 990.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
Biotin | 4.0 | mg | 0.004 | 0.016 | |
Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
Calcium pantothenate | 10.0 | mg | 0.01 | 0.021 | |
Folic acid | 1.0 | mg | 1.0e-3 | 0.002 | |
Riboflavin | 10.0 | mg | 0.01 | 0.027 | |
Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 | |
Distilled water | 1000.0 | ml | - | - | |
1 Filter-sterilized. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Filter |
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