DSMZ 158 . THERMOPLASMA ACIDOPHILUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Selected strain: Thermoplasma acidophilum DSM 1728

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.320 g 1.32 9.989
KH2PO4 0.372 g 0.372 2.734
MgSO4 x 7 H2O 0.247 g 0.247 1.002
CaCl2 x 2 H2O 0.074 g 0.074 0.503
Trace element solution 10.000 ml - -
Yeast extract
(Oxoid)
2.000 g 2 -
Glucose 20.000 g 20 111.012
Distilled water
(freshly distilled)
1000.000 ml - -
1 Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium.
Solution occurs in 1 other media (first defined in Medium 158).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 1.93 g 1.93 7.14
MnCl2 x 4 H2O 0.18 g 0.18 0.91
Na2B4O7 x 10 H2O 0.45 g 0.45 -
ZnSO4 x 7 H2O 22.00 mg 0.022 0.077
CuCl2 x 2 H2O 5.00 mg 0.005 0.029
Na2MoO4 x 2 H2O 3.00 mg 0.003 0.012
VOSO4 x 5 H2O 3.80 mg 0.004 0.015
CoSO4 x 7 H2O 2.00 mg 0.002 0.013
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 1.0
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Associated strains

All strains for this medium: 1
Thermoplasma acidophilum DSM 1728

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 20 111.012
Yeast extract 2 -
(NH4)2SO4 1.32 9.989
KH2PO4 0.372 2.734
MgSO4 x 7 H2O 0.247 1.002
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain