158 .
THERMOPLASMA ACIDOPHILUM MEDIUM
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Selected strain: Thermoplasma acidophilum DSM 1728
This strain uses the standard medium without modifications.
Growth conditions: 55 °C
Equipment needed:
Final pH:
1.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 1.320 | g | ||
|
|
KH2PO4 | 0.372 | g | ||
|
|
MgSO4 x 7 H2O | 0.247 | g | ||
|
|
CaCl2 x 2 H2O | 0.074 | g | ||
|
|
Trace element solution | 10.000 | ml | ||
|
|
Yeast extract
(Oxoid) |
2.000 | g | ||
|
|
Glucose | 20.000 | g | ||
|
|
Distilled water
(freshly distilled) |
1000.000 | ml | ||
| 1 Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeCl3 x 6 H2O | 1.93 | g |
|
|
MnCl2 x 4 H2O | 0.18 | g |
|
|
Na2B4O7 x 10 H2O | 0.45 | g |
|
|
ZnSO4 x 7 H2O | 22.00 | mg |
|
|
CuCl2 x 2 H2O | 5.00 | mg |
|
|
Na2MoO4 x 2 H2O | 3.00 | mg |
|
|
VOSO4 x 5 H2O | 3.80 | mg |
|
|
CoSO4 x 7 H2O | 2.00 | mg |
|
|
Distilled water | 1000.00 | ml |