Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
|Clarified rumen fluid||300.00||ml||-||-|
|CaCl2 x 2 H2O||0.12||g||0.12||0.816|
|MgSO4 x 7 H2O||0.18||g||0.18||0.73|
|L-Cysteine HCl x H2O||1.00||g||1||5.694|
|1 Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH with KOH to 6.5. Distribute medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Therafter, add glucose from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final pH of the medium should be 6.7 - 7.0.|
|1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.|
|Synonyms:||TREPONEMA BRYANTII MEDIUM|
|Medium type:||Complex medium|
|Final pH:||6.7 - 7.0|
|Equipment needed:||Autoclave, Centrifuge, Filter, Gassing station, Hungate tubes|
|Edit in Medium builder|
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|Compound||Conc. [g/L]||Conc. [mM]|
|L-Cysteine HCl x H2O||1||5.694|