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1597. METHANOSALSUM NATRONOPHILUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 750 ml - -
2 Solution B 250 ml - -
3 Solution C 1 ml - -
4 Solution D 1 ml - -
5 Solution E 5 ml - -
6 Solution F 3 ml - -
7 Solution G 5 ml - -
8 Solution H 10 ml - -
9 Solution I 10 ml - -
10 Solution J 10 ml - -
11 , may stimulate growth of some strains 20 mg 0.019 -
Instructions:
  1. Sterilize solutions A and B in closed thick-walled screw-top bottles (e.g., SCHOTT) for 20 min at 120°C. The pH of solution A after sterilization should be 10. There is some precipitate forming that settles at the bottom after 3 - 4 days. It is best to remove precipitates by decantation before using solution A for medium preparation.
  2. Combine solution A with solution B and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions C to J are sterilized separately under 100% N2 gas. Vitamins and coenzyme M should be sterilized by filtration. To complete the medium appropriate amounts of solutions C to J are added to the combined sterile solutions A and B in the sequence as indicated. Final pH of the medium should be 9.5.
  3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 71.25 g 95 896.328
2 11.25 g 15 178.557
3 4.00 g 5.333 91.258
4 0.75 g 1 5.741
5 750.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 120 2053.3
2 0.25 g 1 5.741
3 0.25 g 1 13.414
4 250.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace elements solution (Pfennig & Lippert,1966) 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 40 747.787
2 0.25 g 50 202.86
3 5.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3 ml 790 24655.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.02 g 4 -
2 5.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(coenzyme M)
0.15 g 15 105.486
2 10.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 25 104.088
2 10.00 ml 998.207 55408.8
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 995.341 55249.7
Na2CO3 68.182 643.298
NaCl 32.536 556.716
NaHCO3 10.766 128.151
Methanol 2.268 70.781
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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