Loading ...

1597a. METHANONATRONARCHAEUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution C (ferrous sludge) 100 ml - -
2 Solution A 500 ml - -
3 Solution B 500 ml - -
4 Solution C 1 ml - -
5 Solution D 1 ml - -
6 Solution E 5 ml - -
7 Solution F 2 ml - -
8 Solution G 10 ml - -
9 Solution H 5 ml - -
10 Solution I 10 ml - -
11 Solution J 10 ml - -
12 Solution K 5 ml - -
13 Solution L 10 ml - -
14 , may stimulate growth of some strains 20 mg 0.017 -
Instructions:
  1. Sterilize solutions A and B in closed thick-walled screw-top bottles (e.g., SCHOTT) for 20 min at 120°C. The pH of solution A after sterilization should be 10. There is some precipitate forming that settles at the bottom after 3 - 4 days. It is best to remove precipitates by decantation before using solution A for medium preparation.
  2. Combine solution A with solution B and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions C to L are sterilized separately under 100% N2 gas. Vitamins and coenzyme M should be sterilized by filtration. To complete the medium appropriate amounts of solutions C to L are added to the combined sterile solutions A and B in the sequence as indicated. Final pH of the medium should be 9.5.
  3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 92.5 g 185 1745.48
2 17.5 g 35 416.634
3 8.0 g 16 273.773
4 0.5 g 1 5.741
5 500.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 120.00 g 240 4106.6
2 1.25 g 2.5 14.353
3 2.50 g 5 67.068
4 500.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace elements solution (Pfennig & Lippert,1966) 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 40 747.787
2 0.25 g 50 202.86
3 5.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2 ml 790 24655.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.40 g 340 4999.47
2 0.16 g 16 195.042
3 10.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.02 g 4 -
2 5.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(coenzyme M)
0.15 g 15 105.486
2 10.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution C (ferrous sludge) 5 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 25 104.088
2 10.00 ml 998.207 55408.8
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium 1267 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 15.4 g 154 553.927
2 12.3 g 123 512.112
3 100.0 ml 998.207 55408.8
Instructions:
  1. Heat distilled water to 50°C in a 250 ml beaker with a stir bar present. While rapidly stirring the water, add the ferrous sulfate followed immediately by the sodium sulfide. The formed black FeS sludge is decanted into a glass bottle that can be stoppered. The FeS is allowed to settle for several hours and then the overlying water is decanted and replaced. This procedure is repeated at least five times to wash the FeS. After washing, the pH of the FeS solution should be close to neutrality. The FeS suspension can be kept in closed bottles or tubes under a nitrogen atmosphere for at least three months.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 9.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.484 55313.2
NaCl 110.44 1889.72
Na2CO3 79.81 753.011
NaHCO3 15.099 179.739
FeSO4 x 7 H2O 13.954 50.19
Show more

Cultivation metadata from 1 strains BacDive

halophily extreme 1 strain
halophily moderate 1 strain
pH 10 - 14 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
Show more

Similar media

867. RHODOBACTER VELDKAMPII MEDIUM 93 %
J1180. CELLULOLYTIC NATRONOARCHAEA MEDIUM 92 %
1352. FUCHSIELLA ALKALIACETIGENA MEDIUM 92 %
1095. NATRANAEROBIUS MEDIUM 91 %
784a. METHANOCALCULUS NATRONOPHILUS MEDIUM 91 %

Latest comments

No comments for this medium.