1601
1601 .
MODIFIED M13 VERRUCOMICROBIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1601 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.25 | g | 0.25 | - | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| Glucose | 0.25 | g | 0.25 | 1.388 | |
| Vitamin solution | 10.00 | ml | - | - | |
| Hutner's salts | 20.00 | ml | - | - | |
|
Tris-HCl buffer
(0.1M, pH 7.5) |
50.00 | ml | - | 5 | |
|
Sea water
(Biomaris MeeresTIEFwasser) |
900.00 | ml | - | - | |
| Distilled water | 30.00 | ml | - | - | |
| 1 Autoclave at 121°C for 15 min. | |||||
Solution occurs in 2
other media (first defined in Medium 603).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 | |
| Biotin | 2.0 | mg | 0.002 | 0.008 | |
| Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 | |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 | |
| Folic acid | 2.0 | mg | 0.002 | 0.005 | |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 | |
| Nicotinamide | 5.0 | mg | 0.005 | 0.041 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Filter-sterilized | |||||
Solution occurs in 24
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.000 | ml | - | - | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Metadata
| Last modified: | 15.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| Glucose | 0.25 | 1.388 |
| Peptone | 0.25 | - |
| Yeast extract | 0.25 | - |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Show more | ||
