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DSMZ

DSMZ 1605 . HLH-700 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1605 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 2.609 g 2.609 34.997
NH4Cl 0.267 g 0.267 4.992
MgSO4 x 7 H2O 172.536 g 172.536 700.011
K2HPO4 0.030 g 0.03 0.172
Na2SO4 19.886 g 19.886 140.003
Na2CO3 0.023 g 0.023 0.217
TES 2.292 g 2.292 9.998
Yeast extract 2.500 g 2.5 -
Fe(NH4)citrate 6.000 mg 0.006 -
NaHCO3 0.840 g 0.84 9.999
BG-FPC (100x) 1.000 ml - -
Distilled water 1000.000 ml - -
Wolfe´s Vitamins 1.000 ml - -
1 Adjust pH to 8.0
2 Sterilize at 121°C for 15 minutes. After sterilization add 1 ml/l filter-sterilized Wolfe´s vitamin solution.

Trace minerals (10x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Co(NO3)2 x 6 H2O 0.0494 g 0.049 0.17
CuSO4 x 4 H2O 0.0790 g 0.079 0.495
MnCl2 x 4 H2O 1.8100 g 1.81 9.146
ZnSO4 0.2220 g 0.222 1.375
H3BO3 2.8600 g 2.86 46.248
Na2MoO4 x 2 H2O 0.3900 g 0.39 1.612
Distilled water 1000.0000 ml - -

BG-FPC (100x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 149.580 g 149.58 1759.87
MgSO4 x 7 H2O 7.490 g 7.49 30.388
CaCl2 x 2 H2O 3.600 g 3.6 24.487
Na2-EDTA 0.104 g 0.104 0.309
Trace minerals (10x) 100.000 ml - -
Distilled water 1000.000 ml - -

Wolfe´s Vitamins #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 19.886 140.003
KCl 2.609 34.997
Yeast extract 2.5 -
TES 2.292 9.998
NaHCO3 0.84 9.999
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain