Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Casitone | 3.00 | g | ||
|
CaCl2 x 2 H2O | 1.36 | g | ||
|
Yeast extract | 1.00 | g | ||
|
Agar , alternative | 15.00 | g | ||
|
Cellobiose
, alternative
(filter-sterilized) |
0.50 | % | ||
|
Distilled water | 1000.00 | ml | ||
1 Adjust pH to 7.2. | |||||
2 To medium add 0.5% cellobiose (filter-sterilized) or distribute medium (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium. |