DSMZ 160 . CYTOPHAGA HUTCHINSONII MEDIUM

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Equipment needed:

Final pH: 7.2

Select a strain (optional):

ml
Compound Amount Unit
Casitone 3.00 g
CaCl2 x 2 H2O 1.36 g
Yeast extract 1.00 g
Agar , alternative 15.00 g
Cellobiose , alternative
(filter-sterilized)
0.50 %
Distilled water 1000.00 ml
1 Adjust pH to 7.2.
2 To medium add 0.5% cellobiose (filter-sterilized) or distribute medium (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium.