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DSMZ

DSMZ 1610 . METHYLOCEANIBACTER MEDIUM (NaCl-dANMS)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1610 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (50x salt stock) 20.0 ml - -
Solution 2 (100 mM Fe EDTA) 0.4 ml - -
Solution 3 (100 mM KH2PO4) 10.0 ml - -
Solution 4 (10 mM CuSO4 x 5 H2O) 0.1 ml - -
Solution 5 (30% NaCl) 100.0 ml - -
Solution 6 (100 mM NH4Cl) 20.0 ml - -
Solution 7 (100 mM KNO3) 20.0 ml - -
Solution 8 (100 mM Hepes) 50.0 ml - -
Solution 9 (100µM lanthanides) 1.0 ml - -
Trace element solution 1.0 ml - -
Distilled water 1000.0 ml - -
Methanol
(filter sterilized) 		10.0 ml 7.92 247.176
1 Dilute 20 ml of solution 1 to 700ml with distilled water.
2 Add 100mL of solution 5, 50 mL of solution 8, 20mL of solution 6 and 7 and 10 mL of solution 3.
3 Add 1ml trace element solution.
4 Add 0.4 mL solution 2.
5 Add 0.1 mL solution 4.
6 Add 1.0 mL solution 9.
7 Disolve and bring to 1L.
8 Adjust pH to 7.8 with HCl or NaOH. Autoclave medium.
9 After autoclaving add 10mL per liter of filter sterilized methanol.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.50 g 0.5 1.711
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 0.01 g 0.01 0.062
MnCl2 3.00 mg 0.003 0.024
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.02 g 0.02 0.154
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 2.00 mg 0.002 0.008
Distilled water 1000.00 ml - -
1 May be stored at 4°C in the dark

Solution 1 (50x salt stock) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 6 H2O 10.0 g 10 83.078
CaCl2 x 2 H2O 1.5 g 1.5 10.203
Distilled water 1000.0 ml - -
1 Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre

Solution 2 (100 mM Fe EDTA) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III)-EDTA 36.05 g 36.05 104.779
Distilled water 1000.00 ml - -

Solution 3 (100 mM KH2PO4) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 13.6 g 13.6 99.938
Distilled water 1000.0 ml - -

Solution 4 (10 mM CuSO4 x 5 H2O) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CuSO4 x 5 H2O 2.5 g 2.5 18.371
Distilled water 1000.0 ml - -

Solution 5 (30% NaCl) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30 g 30 513.347
Distilled water 1000 ml - -

Solution 6 (100 mM NH4Cl) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 5.3 g 5.3 99.084
Distilled water 1000.0 ml - -

Solution 7 (100 mM KNO3) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 10.1 g 10.1 99.898
Distilled water 1000.0 ml - -

Solution 8 (100 mM Hepes) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
HEPES 23.8 g 23.8 99.874
Distilled water 1000.0 ml - -

Solution 9 (100µM lanthanides) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CeCl3 x 7 H2O 0.04 g 0.04 0.162
LaCl3 x 7 H2O 0.04 g 0.04 -
NdCl3 x 6 H2O 0.04 g 0.04 -
PrCl3 x H2O 0.02 g 0.02 -
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.8
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Methanol 7.92 247.176
NaCl 3 51.335
HEPES 1.19 4.994
KNO3 0.202 1.998
MgSO4 x 6 H2O 0.2 1.662
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