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DSMZ

DSMZ 1611 . YCFA MEDIUM (modified)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Medium for Clostridium ammoniilyticum DSM 108253

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 37 °C

Medium 1611 in Hungate tube #

ml
Equipment needed: Autoclave Hungate tubes Filter
1 Prepare the Medium (see Main sol. 1611). Distribute under N2 into Hungate tubes (10 ml):
Compound Amount Unit Conc. [g/L] Conc. [mM]
Main sol. 1611 10.0 ml - -
2 Autoclave. Then filter sterilize vitamin solution and aseptically add 100 µl per Hungate tube:
Vitamin solution
(filter-sterilized) 		0.1 ml - -

Main sol. 1611 #

1000
Equipment needed: Gassing station Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casitone 10.00 g 10 -
Yeast extract 2.50 g 2.5 -
Glucose 5.00 g 5 27.753
MgSO4 x 7 H2O 45.00 mg 0.045 0.183
CaCl2 x 2 H2O 90.00 mg 0.09 0.612
K2HPO4 0.45 g 0.45 2.584
KH2PO4 0.45 g 0.45 3.307
NaCl 0.90 g 0.9 15.4
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Dissolve ingredients except NaHCO3, hemin, cysteine in water and boil for 10 min, then add fatty acids:
Volatile fatty acids 2.70 ml - -
2 Cool down to room temperature while gassing with 100% CO2. Add the NaHCO3, hemin, cysteine.
NaHCO3 4.00 g 4 47.615
L-Cysteine HCl 1.00 g 1 6.344
Hemin 10.00 mg 0.01 0.015
3 Adjust pH to 6.7-6.8.

Volatile fatty acids #

3
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acetic acid 1.9 ml 661.833 11021.4
Propionic acid 0.7 ml 231.7 3127.7
iso-Butyric acid 90.0 µl 28.5 323.459
n-Valeric acid 100.0 µl 31.3 306.472
iso-Valeric acid 100.0 µl 31.033 303.861

Vitamin solution #

1000
Equipment needed: Filter Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 17.11.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7 - 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Associated strains

All strains for this medium: 62
Strains with modifications: 6
Clostridium ammoniilyticum DSM 108253

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Casitone 10 -
Glucose 5 27.753
NaHCO3 4 47.615
Yeast extract 2.5 -
Acetic acid 1.787 29.758
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Cultivation metadata from 62 strains BacDive

pH 4 - 6 1 strain
phyla 6 56 strains
oxygen anaerobe 30 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 54 strains