MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1615 . Basic mineral medium

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1615 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
20 mM K-P-Buffer 1000.0 ml - -
1 After sterilization add from sterile stock solutions:
MgSO4 x 7 H2O
(1 M) 		1.0 ml 0.246 1
Sodium acetate
(1 M) 		10.0 ml 0.82 10
CaCl2 x 2 H2O
(5%) 		1.0 ml 0.05 0.34
2 might result in colloid formation, especially when added into hot medium, but dissolves after vigorous shaking
Na-aspartate
(2 M) 		1.0 ml 0.354 2
Trace element solution 1.0 ml - -
L-Methionine
(50 mM, filter sterilized, neutralised) 		1.0 ml 0.007 0.05
Vitamine mixture 0.5 ml - -
3 pH 7.0-7.2

Trace element solution #

1000
Solution occurs in 3 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

20 mM K-P-Buffer #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(0.1 M) 		130 ml 2.264 13
KH2PO4
(0.1 M) 		70 ml 0.953 7
Distilled water 800 ml - -
1 Sterilize at 121°C for 20 minutes in 1L Schott bottle

Vitamine mixture #

400
Solution occurs in 2 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acidic thiamine / Ca pantothenate solution 100 ml - -
Basic vitamin solution 100 ml - -
Neutral folic acid / riboflavin solution 100 ml - -
Neutral B12 solution 0.005 g/L 100 ml - -
1 Sterilize by filtration.
2 Mix the four solutions in the ratio 1:1:1:1.

Acidic thiamine / Ca pantothenate solution #

100
Solution occurs in 2 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine 5 mg 0.05 0.188
Calcium pantothenate 5 mg 0.05 0.105
Distilled water
(acidified to pH 3.0 with HCl) 		100 ml - -

Basic vitamin solution #

100
Solution occurs in 2 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.02 0.082
PABA
(p-aminobenzoic acid) 		5 mg 0.05 0.365
Nicotinic acid 5 mg 0.05 0.406
Pyridoxine 10 mg 0.1 0.591
NaOH
(0.1N) 		100 ml - -

Neutral folic acid / riboflavin solution #

100
Solution occurs in 2 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2 mg 0.02 0.045
Riboflavin 5 mg 0.05 0.133
Distilled water 100 ml - -

Neutral B12 solution 0.005 g/L #

100
Solution occurs in 2 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
B12 0.5 mg 0.005 0.004
Distilled water 100.0 ml - -

Metadata

Last modified: 03.08.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2HPO4 2.264 13
KH2PO4 0.953 7
Sodium acetate 0.82 10
Na-aspartate 0.354 2
MgSO4 x 7 H2O 0.246 1
Show more
CSV
JSON