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DSMZ

DSMZ 1615 . Basic mineral medium

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1615 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
20 mM K-P-Buffer 1000.0 ml - -
1 After sterilization add from sterile stock solutions:
MgSO4 x 7 H2O
(1 M) 		1.0 ml 0.246 1
Sodium acetate
(1 M) 		10.0 ml 0.82 10
CaCl2 x 2 H2O
(5%) 		1.0 ml 0.05 0.34
2 might result in colloid formation, especially when added into hot medium, but dissolves after vigorous shaking
Na-aspartate
(2 M) 		1.0 ml 0.354 2
Trace element solution 1.0 ml - -
L-Methionine
(50 mM, filter sterilized, neutralised) 		1.0 ml 0.007 0.05
Vitamine mixture 0.5 ml - -
3 pH 7.0-7.2

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 1478).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles

20 mM K-P-Buffer #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(0.1 M) 		130 ml 2.264 13
KH2PO4
(0.1 M) 		70 ml 0.953 7
Distilled water 800 ml - -
1 Sterilize at 121°C for 20 minutes in 1L Schott bottle

Vitamine mixture #

400
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acidic thiamine / Ca pantothenate solution 100 ml - -
Basic vitamin solution 100 ml - -
Neutral folic acid / riboflavin solution 100 ml - -
Neutral B12 solution 0.005 g/L 100 ml - -
1 Sterilize by filtration.
2 Mix the four solutions in the ratio 1:1:1:1.

Acidic thiamine / Ca pantothenate solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine 5 mg 0.05 0.188
Calcium pantothenate 5 mg 0.05 0.105
Distilled water
(acidified to pH 3.0 with HCl) 		100 ml - -

Basic vitamin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.02 0.082
PABA
(p-aminobenzoic acid) 		5 mg 0.05 0.365
Nicotinic acid 5 mg 0.05 0.406
Pyridoxine 10 mg 0.1 0.591
NaOH
(0.1N) 		100 ml - -

Neutral folic acid / riboflavin solution #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2 mg 0.02 0.045
Riboflavin 5 mg 0.05 0.133
Distilled water 100 ml - -

Neutral B12 solution 0.005 g/L #

100
Solution occurs in 4 other media (first defined in Medium 1615).
Compound Amount Unit Conc. [g/L] Conc. [mM]
B12 0.5 mg 0.005 0.004
Distilled water 100.0 ml - -

Metadata

Last modified: 03.08.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2HPO4 2.264 13
KH2PO4 0.953 7
Sodium acetate 0.82 10
Na-aspartate 0.354 2
MgSO4 x 7 H2O 0.246 1
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