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DSMZ

DSMZ 1622 . RUGOSIBACTER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1622 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
Vitamin B12
(50 mg/l) 		1 ml - -
1 Adjust solutions A and B to pH 7.0±0.2, autoclave separately and combine after cooling.
2 Add 1.0 ml sterile vitamin B12 solution (50 mg/l) per litre.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.12 g 0.24 1.764
Na2HPO4 x 2 H2O 0.72 g 1.44 10.144
Distilled water 500.00 ml - -

Solution B #

500
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4NO3 0.40 g 0.8 -
MgSO4 x 7 H2O 0.24 g 0.48 1.947
CaCl2 x 2 H2O 0.15 g 0.3 2.041
Na-pyruvate 2.00 g 4 36.349
Trace element solution SL-10 1.00 ml - -
Distilled water 500.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-pyruvate 2 18.175
Na2HPO4 x 2 H2O 0.72 5.072
NH4NO3 0.4 -
MgSO4 x 7 H2O 0.24 0.974
CaCl2 x 2 H2O 0.15 1.02
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 2 strains
phyla 1 2 strains
nutrition heterotroph 2 strains
oxygen aerobe 2 strains
temperature mesophilic 2 strains