1629 .
MINIMAL MEDIA CONTAINING METHANOL
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Equipment needed:
Autoclave
Filter
Final pH:
7.1
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 1.62 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
K2HPO4 | 2.40 | g | ||
|
|
NaH2PO4 x 2 H2O | 1.10 | g | ||
|
|
Na2-EDTA | 15.00 | mg | ||
|
|
FeSO4 x 6 H2O | 3.00 | mg | ||
|
|
ZnSO4 x 7 H2O | 4.50 | mg | ||
|
|
CoCl2 x 6 H2O | 3.00 | mg | ||
|
|
MnCl2 x 4 H2O | 0.64 | mg | ||
|
|
H3BO3 | 1.00 | mg | ||
|
|
Na2MoO4 x 2 H2O | 0.40 | mg | ||
|
|
CuSO4 x 2 H2O | 0.30 | mg | ||
|
|
CaCl2 x 6 H2O | 3.00 | mg | ||
|
|
Methanol | 5.00 | ml | ||
|
|
Agar | 15.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the medium without methanol. Adjust pH to 7.1. | |||||
| 2 Autoclave and after cooling to 50°C add the methanol from a filter-sterilized stock solution. | |||||