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DSMZ

DSMZ 1634 . SERPENTINOMONAS MINIMAL MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1634 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 28.40 mg 0.028 0.2
MgCl2 x 6 H2O 40.40 mg 0.04 0.199
NH4Cl 80.20 mg 0.08 1.499
CAPS buffer 3.32 g 3.32 15.001
Agar , if required
(washed Noble-Agar)
optional 		15.00 g - -
Double distilled water 1000.00 ml - -
1 Adjust pH to 11.0 with NaOH.
2 Fill into screw-cap tubes, leaving 60% headspace.
3 Sparge medium with 100% N2 gas mixture to make it anoxic.
4 Autoclave at 121 °C for 20 min.
5 After autoclaving, cool down and add from sterile stock solutions while stirring:
K2HPO4
(1ml of 300 mM stock solution per liter) 		0.30 mM 0.052 0.3
CaCl2
(5ml of 1 M stock solution per liter) 		5.00 mM 0.735 5
Na acetate
(2ml of 2 M stock solution per liter) 		4.00 mM 0.328 4
Vitamin solution 10.00 ml/l - -
Trace element solution 10.00 ml/l - -
6 Add 30% air.
7 Note: Some precipitate will form.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2.0 mg 0.002 0.005
Biotin 2.0 mg 0.002 0.008
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Thiamine 5.0 mg 0.005 0.019
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Thioctic acid 5.0 mg 0.005 0.024
Monopotassium Phosphate 900.0 mg 0.9 6.614
Distilled water 1000.0 ml - -

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA x 2 H2O 500 mg 0.5 1.487
MgSO4 x 7 H2O 3000 mg 3 12.172
MnSO4 x H2O 500 mg 0.5 2.958
NaCl 1000 mg 1 17.112
FeSO4 x 7 H2O 100 mg 0.1 0.36
Co(NO3)2 x 6 H2O 100 mg 0.1 0.344
CaCl2
(anhydrous) 		100 mg 0.1 0.901
ZnSO4 x 7 H2O 100 mg 0.1 0.348
CuSO4 x 5 H2O 10 mg 0.01 0.04
AlK(SO4)2
(anhydrous) 		10 mg 0.01 0.039
H3BO3 10 mg 0.01 0.162
Na2 MoO4 x 2 H2O 10 mg 0.01 0.041
Na2SeO3
(anhydrous) 		1 mg 1.0e-3 0.006
Na2WO4 x 2 H2O 10 mg 0.01 0.03
NiCl2 x 6 H2O 20 mg 0.02 0.154
Distilled water 1000 ml - -

Metadata

Last modified: 07.07.22
Source: DSMZ
Medium type: Defined medium
Final pH: 11.0
Equipment needed: Autoclave
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Gas composition

  • 70 % N2
  • 30 % Air

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
CAPS buffer 3.32 15.001
CaCl2 x 2 H2O 0.735 5
Na acetate 0.328 4
NH4Cl 0.08 1.499
K2HPO4 0.052 0.3
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains