1639
1639 .
AZOARCUS TAIWANENSIS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1639 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 10.00 | g | 10 | 171.116 | |
| KNO3 | 0.76 | g | 0.76 | 7.517 | |
| NH4Cl | 0.10 | g | 0.1 | 1.87 | |
| K2HPO4 | 0.25 | g | 0.25 | 1.435 | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
| NaHCO3 | 1.50 | g | 1.5 | 17.856 | |
| Na-acetate | 0.44 | g | 0.44 | 5.364 | |
| Na2S x 9 H2O | 0.10 | g | 0.1 | 0.416 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, acetate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then, add and dissolve bicarbonate, adjust pH to 8.5 - 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add acetate and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Adjust pH of complete medium to 8.5 - 9.0, if necessary. | |||||
Solution occurs in 22
other media (first defined in Medium 722).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Metadata
| Last modified: | 12.05.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 8.5 - 9.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 10 | 171.116 |
| NaHCO3 | 1.5 | 17.856 |
| KNO3 | 0.76 | 7.517 |
| Na-acetate | 0.44 | 5.364 |
| K2HPO4 | 0.25 | 1.435 |
| Show more | ||
