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DSMZ

DSMZ 1647 . ACETOBACTEROIDES MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Acetobacteroides hydrogenigenes DSM 24657

This strain uses the standard medium without modifications.

Main sol. 1647 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.20 g 0.2 -
Glucose 0.18 g 0.18 0.999
KH2PO4 0.14 g 0.14 1.029
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.15 g 0.15 1.02
NH4Cl 0.54 ml 0.825 15.42
L-Cysteine HCl x H2O 0.30 g 0.3 1.708
NaHCO3 2.50 g 2.5 29.759
Na2S x 9 H2O 0.30 g 0.3 1.249
Resazurin 1.00 mg 1.0e-3 0.004
Vitamin solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 The vitamin solution, trace element solution, Se/W solution, cysteine, glucose, Na2S and NaHCO3 are added after the medium has been autoclaved.

Se/W solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 4 mg 0.004 0.023
Na2WO4 4 mg 0.004 0.014
Distilled water 1000 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
1 Note: for preparation see Medium 141

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.80 g 12.8 66.967
FeCl2 x 4 H2O 1.00 g 1 5.03
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.03 g 0.03 0.231
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiCl2 x 6 H2O 0.10 g 0.1 0.772
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Na2WO4 x 2 H2O 0.04 g 0.04 0.121
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.

Metadata

Last modified: 01.01.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1
Acetobacteroides hydrogenigenes DSM 24657

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.5 29.759
NH4Cl 0.825 15.42
L-Cysteine HCl x H2O 0.3 1.708
Na2S x 9 H2O 0.3 1.249
MgCl2 x 6 H2O 0.2 0.984
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain