1650
1650 .
1650: Medium for Strain J5B
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1650 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2S2O3 x 5 H2O | 2.50 | g | 2.5 | 10.073 | |
| NaNO3 | 0.85 | g | 0.85 | 10.001 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
| NH4Cl | 0.10 | g | 0.1 | 1.87 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Trace Element Solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH 7.0-7.2. | |||||
| 2 Dispense the medium into sterile bottles. Fill 6 ml medium in 15 ml tubes, fill the headspace with a mixture of N2/CO2 (80:20) and seal the bottles with butyl rubber stoppers . Autoclave at 121°C for 15 min. Add following solutions aseptically: 1 M Bicarbonate solution (see below) 30 ml, Vitamin solution (see below) 1 ml, 1 M acetate solution (see below) 1 ml. Adjust pH 7.0-7.2 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.500 | ml | 3.125 | 85.71 |
| FeSO4 x 7 H2O | 2.100 | g | 2.075 | 7.464 |
| ZnSO4 x 7 H2O | 0.144 | g | 0.142 | 0.495 |
| MnCl2 x 4 H2O | 0.100 | g | 0.099 | 0.499 |
| H3BO3 | 0.030 | g | 0.03 | 0.479 |
| CoCl2 x 6 H2O | 0.190 | g | 0.188 | 1.446 |
| CuCl2 x 2 H2O | 2.000 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 0.024 | g | 0.024 | 0.183 |
| Na2MoO4 x 2 H2O | 0.036 | g | 0.036 | 0.147 |
| Distilled water | 1000.000 | ml | - | - |
Solution occurs in 71
other media (first defined in Medium J431).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.4 | g | 0.4 | 10.001 |
| Na2SeO3 x 5 H2O | 6.0 | mg | 0.006 | 0.023 |
| Na2WO4 x 2 H2O | 8.0 | mg | 0.008 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sodium acetate trihydrate | 13.6 | g | 13.6 | 99.941 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH 7.0-7.2 and autoclave at 121°C for 15 min. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 8.4 | g | 84 | 999.917 | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Saturated with CO2 gas and tightly sealed in bottles filled with CO2 gas. Autoclave at 121°C for 15 min | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 | |
| Folic acid | 2.0 | mg | 0.002 | 0.005 | |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 | |
| Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 | |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 | |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 | |
| D-Calcium pantothenate | 5.0 | mg | 0.005 | - | |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 | |
| 4-Aminobenzoic Acid | 5.0 | mg | 0.005 | 0.036 | |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Filter-sterilized the vitamin solution | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .