1656
1656 .
HALANAEROBIUM HYDROGENIFORMANS MEDIUM (SL-HP)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1656 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 70.00 | g | 3500 | 59890.5 | |
| Na2CO3 | 40.00 | g | 2000 | 18870.1 | |
| K2HPO4 | 6.30 | g | 315 | 1808.52 | |
| Yeast extract | 1.00 | g | 50 | - | |
| Na2S x 9 H2O | 0.75 | g | 37.5 | 156.132 | |
| L-Cysteine | 0.60 | g | 30 | 247.606 | |
| Basal medium stock solution | 10.00 | ml | - | - | |
| Trace mineral solution | 10.00 | ml | - | - | |
|
Na2S x 9 H2O
(10% v/v) |
5.00 | ml/l | - | - | |
|
L-Cysteine
(5% v/v) |
12.00 | ml/l | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without cysteine hydrochloride, Na2S x 9 H2O and Na2CO3. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the Na2CO3. Autoclave the medium and add from sterile stock solutions cysteine hydrochloride and Na2S x 9 H2O. The final pH should be 11.0. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NH4NO3 | 50.0 | mg | 0.05 | - |
| MgCl2 x 6 H2O | 8.5 | mg | 0.009 | 0.042 |
| SiO2 | 7.5 | mg | 0.008 | 0.125 |
| MnSO4 x H2O | 4.5 | mg | 0.005 | 0.027 |
| CaCl2 x 2 H2O | 4.2 | mg | 0.004 | 0.029 |
| FeSO4 x 7 H2O | 1.8 | mg | 0.002 | 0.006 |
| Resazurin | 4.0 | mg | 0.004 | 0.017 |
| Distilled water | 1000.0 | ml | - | - |
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| MgSO4 x 7 H2O | 3.000 | g | 2.941 | 11.933 |
| Na3-NTA x H2O | 1.630 | g | 1.598 | - |
| NaCl | 1.000 | g | 0.98 | 16.776 |
| MnCl2 x 4 H2O | 0.640 | g | 0.627 | 3.17 |
| ZnCl2 | 0.130 | g | 0.127 | 0.935 |
| FeSO4 x 7 H2O | 0.100 | g | 0.098 | 0.353 |
| CaCl2 x 2 H2O | 0.100 | g | 0.098 | 0.667 |
| NiSO4 x 6 H2O | 0.030 | g | 0.029 | 0.19 |
| Na2MoO4 x 2 H2O | 0.025 | g | 0.025 | 0.101 |
| Na2MoO4 x 2 H2O | 0.025 | g | 0.025 | 0.101 |
| AlK(SO4)2 x 12 H2O | 0.010 | g | 0.01 | 0.021 |
| H3BO3 | 0.010 | g | 0.01 | 0.159 |
| CuCl2 x 2 H2O | 7.000 | mg | 0.007 | 0.04 |
| Distilled water | 1000.000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 11.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .