DSMZ 1662 . ALKALIPHILUS HYDROTHERMALIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.294 2.161
K2HPO4 0.3 g 0.294 1.689
NH4Cl 0.5 g 0.49 9.164
KCl 0.1 g 0.098 1.315
NaCl 5.0 g 4.902 83.88
MgCl2 x 6 H2O 0.5 g 0.49 2.411
CaCl2 x 2 H2O 0.1 g 0.098 0.667
Yeast extract
(OXOID)
5.0 g 4.902 -
Trypticase peptone
(BD BBL)
5.0 g 4.902 -
Modified Wolin's mineral solution 10.0 ml - -
Na2CO3 3.0 g 2.941 27.75
Na-crotonate solution (1 M) 10.0 ml - -
Na2S x 9 H2O 0.3 g 0.294 1.225
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, crotonate and sulfide), adjust pH to 8.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add crotonate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. The crotonate solution should be sterilized by filtration. Adjust pH of the complete medium to 9.0, if necessary.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 3 other media (first defined in Medium 870).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Crotonic acid
(ALDRICH 113018)
86 g 86 998.955
NaOH
(10 N)
100 ml - -
Distilled water 900 ml - -
1 Dissolve crotonic acid in 800 ml distilled water, add around 100 ml of 10 N NaOH and adjust pH to around 7. Then add water to reach a volume of 1000 ml. Sterilize by filtration under 100% N2 gas atmosphere.

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 4.902 -
Trypticase peptone 4.902 -
Na2CO3 2.941 27.75
Crotonic acid 0.843 9.794
NH4Cl 0.49 9.164
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain