DSMZ 1663 . BSM medium

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 3.62 g 3.62 61.944
K2HPO4 0.26 g 0.26 1.493
(NH4)Cl 0.08 g 0.08 1.496
NaNO3 0.85 g 0.85 10.001
Na2SO4 0.05 g 0.05 0.352
Trace element solution 1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Na-DL-lactate 1.12 g 1.12 9.995
Yeast extract 0.20 g 0.2 -
Distilled water 936.50 ml - -
NaHCO3
(8%)
52.50 ml 4.2 49.996
1 Dissolve ingredients (except vitamins and trace elements) in distilled water; boil for 1- 2 minutes and cool down to room temperature under 100% N2 gas, dispense in culture vessels and autoclave. After sterilization add NaHCO3, vitamins, and trace elements from sterile anoxic stock solutions prepared under 100% N2. Adjust pH to 8.2 and add sodium sulfide (Na2S) from a sterile anoxic solution to achieve a final concentration of 100 µM prior inoculation.
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%; 7.7 M)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
MgCl2 x 6 H2O 3.0 g 3 14.756
CaCl2 x 2 H2O 0.1 g 0.1 0.68
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 27.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.2 49.996
NaCl 3.62 61.944
Na-DL-lactate 1.12 9.995
NaNO3 0.85 10.001
K2HPO4 0.26 1.493
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain