DSMZ 1664 . Sphaerotilus natans subspec. sulfidivorans medium

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)Cl 0.30 g 0.3 5.609
KH2PO4 9.00 mg 0.009 0.066
K2HPO4 22.00 mg 0.022 0.126
Na2HPO4 x 7 H2O 35.00 mg 0.035 0.247
MgSO4 x 7 H2O 23.00 mg 0.023 0.093
CaCl2 28.00 mg 0.028 0.252
FeCl3 x 6 H2O 0.25 mg 2.5e-4 9.2e-4
Na-lactate 200.00 mg 0.2 1.785
Peptone 500.00 mg 0.5 -
Na2S2SO3 1.00 g 1 -
Trace element solution 25.00 μl - -
Distilled water 1000.00 ml - -
Standard vitamin solution 25.00 μl - -
1 Dissolve ingredients (except vitamins) in distilled water.
2 After sterilization add the 25 µl vitamin solution.
Solution occurs in 1 other media (first defined in Medium 1664).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.20 g 0.2 0.684
FeSO4 x 7 H2O 0.70 g 0.7 2.518
ZnSO4 x 7 H2O 0.01 g 0.01 0.035
MnSO4 x 4 H2O 2.00 mg 0.002 0.009
CuSO4 x 5 H2O 5.00 mg 0.005 0.02
H3BO3 0.01 g 0.01 0.162
Co(NO3)2 1.00 g 1 5.466
Na2MoO4 x 2 H2O 1.00 g 1 4.133
Distilled water 1000.00 ml - -
Solution occurs in 3 other media (first defined in Medium 81).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Distilled water 100.0 ml - -

Metadata

Last modified: 12.01.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2SO3 1 -
Peptone 0.5 -
(NH4)Cl 0.3 5.609
Na-lactate 0.2 1.785
Na2HPO4 x 7 H2O 0.035 0.247
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain