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DSMZ

DSMZ 1671 . ACIDICAPSA MEDIUM (GYS MEDIUM)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1671 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
1 SOLUTION B
2 Adjust the pH with 1N H2SO4 to 4.5
3 Autoclave solution A and B separately in order to prevent hydrolysis at low pH.

TRACE ELEMENT SOLUTION #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.00 g 5 14.872
FeCl2 x 4 H2O 1.00 g 1 5.03
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.11 g 0.11 0.847
ZnCl2 0.05 g 0.05 0.367
MnCl2 x 4 H2O 0.03 g 0.03 0.152
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiSO4 x 6 H2O 0.03 g 0.03 0.194
CuCl2 x 2 H2O 0.01 g 0.01 0.059
Distilled water 1000.00 ml - -

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.000 g 2 14.697
(NH4)2SO4 1.000 g 2 15.136
Yeast extract 0.300 g 0.6 -
MgCl2 x 6 H2O 0.200 g 0.4 1.968
CaCl2 0.045 g 0.09 0.811
NaCl 0.200 g 0.4 6.845
TRACE ELEMENT SOLUTION 1.000 ml - -
Distilled water 500.000 ml - -

Solution B #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 2 g 4 22.203
Agar , for solid medium 20 g 40 118.931
Distilled water 500 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 4.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.466
Glucose 2 11.101
(NH4)2SO4 1 7.568
KH2PO4 1 7.348
Yeast extract 0.3 -
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain