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1675. DESULFOTHERMOBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.329 6.151
2 0.33 g 0.329 4.413
3 0.33 g 0.329 1.618
4 0.33 g 0.329 2.238
5 0.33 g 0.329 2.418
6 2.80 g 2.792 19.653
7 Trace element solution SL-10 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9 Vitamin solution 1.00 ml - -
10 0.50 g 0.499 2.076
11 1000.00 ml 995.221 55243.1
Instructions:
  1. Dissolve all ingredients except vitamins and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 4.5 with HCl, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 4.5 - 4.8, if necessary.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 4.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
Na2SO4 2.792 19.653
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.329 6.151
KCl 0.329 4.413
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 2 - 4 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
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