DSMZ 1676 . Cyanobacteria Medium ASN3 -

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl
(250 g/l stock solution)
100.0 g/l 100 1711.16
MgCl2 x 6 H2O
(200 g/l stock solution)
10.0 g/l 10 49.188
KCl
(50 g/l stock solution)
10.0 g/l 10 134.138
Mg(SO4) x 7 H2O
(350 g/l stock solution)
10.0 g/l 10 -
CaCl2 x 2 H2O
(50 g/l stock solution)
10.0 g/l 10 68.02
Na3-citrate x 2 H2O
(0.6 g/l stock solution)
5.0 ml/l 0.003 -
Na2-EDTA x 2 H2O
(0.1 g/l stock solution)
5.0 ml/l 5.0e-4 0.001
T-Trace Metal Mix 1.0 ml - -
Distilled water 1000.0 ml - -
1 Adjust to 1000 mL with H2O dist. and autoclave.
2 After cooling, add the following filter sterilized components:
K2HPO4 x 3 H2O
(8.0 g/l stock solution)
2.5 ml/l 0.02 0.115
Na2CO3
(20 g/l stock solution)
1.0 ml/l 0.02 0.189
Fe(NH4)citrate
(6.0 g/l stock solution)
0.5 ml/l 0.003 -
Vitamin B12
(0.02 g/l stock solution)
1.0 ml/l 2.0e-5 1.5e-5
Solution occurs in 1 other media (first defined in Medium 1673).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 2.90 g/l 2.9 46.895
MnCl2 x 4 H2O 1.81 g/l 1.81 9.146
ZnSO4 x 7 H2O 0.22 g/l 0.22 0.765
Na2MoO4 x 2 H2O 0.39 g/l 0.39 1.612
CuSO4 x 5 H2O
(0.08 g/l stock solution)
10.00 ml/l 8.0e-4 0.003
Co(NO3)2 x 6 H2O
(0.05 g/l stock solution)
10.00 ml/l 5.0e-4 0.002

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
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Associated strains

All strains for this medium: 3
Strains with modifications: 3

Light

3 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
KCl 10 134.138
CaCl2 x 2 H2O 10 68.02
MgCl2 x 6 H2O 10 49.188
Mg(SO4) x 7 H2O 10 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature psychrophilic 3 strains