DSMZ 1680 . Cyanobacteria Medium MCL

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial Sea Salt
(hw Marinemix 21010; Wiegandt GmbH – Products for aquaristics, Sterkenhofweg 13, 47807 Krefeld, Germany)
33 g 33 -
Stock Solution A 5 ml - -
Stock Solution B 5 ml - -
1 Adjust to 1000 ml with H2O dist. and autoclave (121°C, 20 min)
2 After cooling add the following filter sterilized component under sterile conditions:
MCL Vitamin Stock Solution 1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
CuSO4 x 7 H2O
(192 mg/100 ml)
5 ml - -
MnSO4 x H2O
(240 mg/100 ml)
100 ml - -
ZnSO4 x 7 H2O
(88 mg/200 ml)
100 ml - -
Fe(NH4)2(SO4)2 x 6 H2O
(598 mg/100 ml)
100 ml - -
1 Adjust to 500 ml with MilliQ water.
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3
(96 mg/100 ml)
100 ml - -
H3BO3
(2240 mg/100 ml)
100 ml - -
NaNO3
(8400 mg/100 ml)
100 ml - -
Na2-EDTA x 2 H2O
(2530 mg/100 ml)
100 ml - -
Sodium beta-glycerophosphate
(800 mg/100 ml)
100 ml - -
1 Adjust to 500 ml with MilliQ water.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12
(1 mg/ml)
160 μl - -
Biotin
(1 mg/ml)
160 μl - -
Thiamine
(10 mg/ml)
800 μl - -
1 Adjust to 100 ml with MilliQ water.

Metadata

Last modified: 02.04.22
Source: DSMZ
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Artificial Sea Salt 33 -
CuSO4 x 7 H2O - -
MnSO4 x H2O - -
ZnSO4 x 7 H2O - -
Fe(NH4)2(SO4)2 x 6 H2O - -
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Cultivation metadata from 0 strains BacDive

phyla 1 4 strains
temperature psychrophilic 4 strains