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DSMZ

DSMZ 1680 . Cyanobacteria Medium MCL

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Equipment needed:
Final pH: n.d.
Description: Provasoli´s Enriched Seawater (PES) based medium for Cyanobacteria adjusted to 28 PSU at 25 °C. [Provasoli L (1968) Media and products for the cultivation of marine algae. In: Watanabe A and Hattori A (eds). Cultures and Collections of Algae (pp. 63-65) Japanese Society of Plant Physiology, Tokyo.]
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ml
Compound Amount Unit
Artificial Sea Salt
(hw Marinemix 21010; Wiegandt GmbH – Products for aquaristics, Sterkenhofweg 13, 47807 Krefeld, Germany) 		33 g
MCL Stock Solution A * 5 ml
MCL Stock Solution B ** 5 ml
1 Adjust to 1000 ml with MilliQ water and autoclave (121°C, 20 min).
2 After cooling add the following filter sterilized component under sterile conditions:
MCL Vitamin Stock Solution *** 1 ml

MCL Stock Solution A * #

500
Compound Amount Unit
CuSO4 x 7 H2O
(1.92 g/l stock solution) 		5 ml
MnSO4 x H2O
(2.4 g/l stock solution) 		100 ml
ZnSO4 x 7 H2O
(0.44 g/l stock solution) 		100 ml
Fe(NH4)2(SO4)2 x 6 H2O
(5.6 g/l stock solution) 		100 ml
1 Adjust to 500 ml with MilliQ water.

MCL Stock Solution B ** #

500
Compound Amount Unit
FeCl3
(0.96 g/l stock solution) 		100 ml
H3BO3
(22.4 g/l stock solution) 		100 ml
NaNO3
(84.0 g/l stock solution) 		100 ml
Na2-EDTA x 2 H2O
(25.3 g/l stock solution) 		100 ml
Sodium beta-glycerophosphate
(8.0 g/l stock solution) 		100 ml
1 Mix to obtain 500 ml of MCL Stock Solution B.

MCL Vitamin Stock Solution *** #

100
Compound Amount Unit
Vitamin B12
(1.0 mg/ml stock solution) 		160 μl
Biotin
(1.0 mg/ml stock solution) 		160 μl
Thiamine
(10.0 mg/ml stock solution) 		800 μl
1 Adjust to 100 ml with MilliQ water.