Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Carboxymethylcellulose
, sodium salt
(CMC) |
2.50 | g | 2.5 | - | |
(NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
KH2PO4 | 0.37 | g | 0.37 | 2.719 | |
Glucose | 1.00 | g | 1 | 5.551 | |
Yeast extract | 0.25 | g | 0.25 | - | |
Tryptone | 0.90 | g | 0.9 | 12.662 | |
MgSO4 x 7 H2O
(25%) |
1.00 | ml | 0.25 | 1.014 | |
CaCl2 x 2 H2O
(7%) |
1.00 | ml | 0.07 | 0.476 | |
FeCl3 x 6 H2O
(2%) |
1.00 | ml | 0.02 | 0.074 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust to pH 4.8-5.0 (liquid medium) and pH 5.5 - 6.0 agar medium. Autoclave MgSO4 x 7 H2O solution, CaCl2 x 2 H2O solution and FeCl3 x 6 H2O solution separately. | |||||
2 Aseptically add all solutions to the basal medium. |
Last modified: | 27.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Carboxymethylcellulose | 2.5 | - |
(NH4)2SO4 | 1.3 | 9.838 |
Glucose | 1 | 5.551 |
Tryptone | 0.9 | 12.662 |
KH2PO4 | 0.37 | 2.719 |
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