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DSMZ

DSMZ 1689 . MEDIUM for SM250

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1689 #

ml
Equipment needed: Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 0.330 g 0.33 1.623
NH4Cl 0.330 g 0.33 6.169
KCl 0.330 g 0.33 4.427
CaCl2 x 2 H2O 0.033 g 0.033 0.224
KH2PO4 0.165 g 0.165 1.212
NaCl 10.000 g 10 171.116
Trace elements solution 1.000 ml - -
Distilled water 1000.000 ml - -
1 Boil the medium for 5 min and cool on ice under N2 (100%). Do not bubble.
NaHCO3 2.000 g 2 23.808
2 pH Wert 7.6-8.0.
3 Fill 10 ml in Hungate tubes with a rubber septum under N2 (100%) flux.
4 Flush each Hungate tube with molecular hydrogen for 10 seconds.
5 Autoclave at 121° for 15 min. After autoclaving add 0.01 ml of filter-sterilized vitamin solution per 10 ml media.
6 Inject 3 ml of sterile air with sterile syringe into each Hungate tube before inoculation.

Trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(NH4)2(SO4)2 x 6 H2O 784 mg 0.784 -
NiCl2 x 2 H2O 284 mg 0.284 2.191
CoCl2 x 6 H2O 28 mg 0.028 0.216
NiCl2 x 2 H2O 166 mg 0.166 1.281
MnCl2 x 4 H2O 99 mg 0.099 0.5
ZnSO4 x 7 H2O 144 mg 0.144 0.501
Na2SeO4 95 mg 0.095 0.503
Na2WO4 x 2 H2O 33 mg 0.033 0.1
Na2MoO4 x 2 H2O 24 mg 0.024 0.099
H3BO3 6 mg 0.006 0.097
CuCl2 x 2 H2O 2 mg 0.002 0.012

Vitamins solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride
(B6) 		100 mg 0.1 0.486
Riboflavine
(B2) 		50 mg 0.05 0.133
Thiamine
(B1) 		50 mg 0.05 0.188
Nicotinic acid
(PP) 		50 mg 0.05 0.406
Pantothenic acid 50 mg 0.05 0.228
Cobalamin
(B12) 		1 mg 1.0e-3 -
p-Aminobenzoic acid 50 mg 0.05 0.365
Lipoic acid 50 mg 0.05 0.242

Metadata

Last modified: 15.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
NaHCO3 2 23.808
NH4Cl 0.33 6.169
KCl 0.33 4.427
MgCl2 x 6 H2O 0.33 1.623
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