This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgCl2 x 6 H2O | 0.330 | g | 0.33 | 1.623 | |
NH4Cl | 0.330 | g | 0.33 | 6.169 | |
KCl | 0.330 | g | 0.33 | 4.427 | |
CaCl2 x 2 H2O | 0.033 | g | 0.033 | 0.224 | |
KH2PO4 | 0.165 | g | 0.165 | 1.212 | |
NaCl | 10.000 | g | 10 | 171.116 | |
Trace elements solution | 1.000 | ml | - | - | |
Distilled water | 1000.000 | ml | - | - | |
1 Boil the medium for 5 min and cool on ice under N2 (100%). Do not bubble. | |||||
NaHCO3 | 2.000 | g | 2 | 23.808 | |
2 pH Wert 7.6-8.0. | |||||
3 Fill 10 ml in Hungate tubes with a rubber septum under N2 (100%) flux. | |||||
4 Flush each Hungate tube with molecular hydrogen for 10 seconds. | |||||
5 Autoclave at 121° for 15 min. After autoclaving add 0.01 ml of filter-sterilized vitamin solution per 10 ml media. | |||||
6 Inject 3 ml of sterile air with sterile syringe into each Hungate tube before inoculation. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Fe(NH4)2(SO4)2 x 6 H2O | 784 | mg | 0.784 | - |
NiCl2 x 2 H2O | 284 | mg | 0.284 | 2.191 |
CoCl2 x 6 H2O | 28 | mg | 0.028 | 0.216 |
NiCl2 x 2 H2O | 166 | mg | 0.166 | 1.281 |
MnCl2 x 4 H2O | 99 | mg | 0.099 | 0.5 |
ZnSO4 x 7 H2O | 144 | mg | 0.144 | 0.501 |
Na2SeO4 | 95 | mg | 0.095 | 0.503 |
Na2WO4 x 2 H2O | 33 | mg | 0.033 | 0.1 |
Na2MoO4 x 2 H2O | 24 | mg | 0.024 | 0.099 |
H3BO3 | 6 | mg | 0.006 | 0.097 |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride
(B6) |
100 | mg | 0.1 | 0.486 |
Riboflavine
(B2) |
50 | mg | 0.05 | 0.133 |
Thiamine
(B1) |
50 | mg | 0.05 | 0.188 |
Nicotinic acid
(PP) |
50 | mg | 0.05 | 0.406 |
Pantothenic acid | 50 | mg | 0.05 | 0.228 |
Cobalamin
(B12) |
1 | mg | 1.0e-3 | - |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
Lipoic acid | 50 | mg | 0.05 | 0.242 |
Last modified: | 15.02.22 |
Source: | DSMZ |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter, Hungate tubes |
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