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DSMZ 1691 . Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D-Glucose 2.5 g 2.5 13.877
Agar
(Bacto)
20.0 g 20 59.465
Artificial Sea Water Solution 2x concentrated (ASW)
(or Biomaris)
1000.0 ml - -
1 Adjust the pH to 7.3 with NaOH 4M
2 Add to 1000 ml of medium after autoclaving and cooling to 55°C:
Trace element solution SL-10 1.0 ml - -
Vitamin solution 1.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.20 g 27.2 465.435
MgCl2 x 6 H2O 9.06 g 9.06 44.564
CaCl2 x 2 H2O 2.60 g 2.6 17.685
KCl 1.28 g 1.28 17.17
MgSO4 x 7 H2O 11.88 g 11.88 48.199
Na2HPO4 x 2 H2O 0.02 g 0.02 0.141
NH4NO3 4.20 mg 0.004 -
Double distilled water 1000.00 ml - -
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 27.2 465.435
Agar 20 59.465
Trypticase peptone 17 -
MgSO4 x 7 H2O 11.88 48.199
MgCl2 x 6 H2O 9.06 44.564
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain

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