This medium was extracted automatically and has not been curated by experts, yet.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase peptone | 17.0 | g | 17 | - | |
Soy peptone | 3.0 | g | 3 | - | |
D-Glucose | 2.5 | g | 2.5 | 13.877 | |
Agar
(Bacto) |
20.0 | g | 20 | 59.465 | |
Artificial Sea Water Solution 2x concentrated (ASW)
(or Biomaris) |
1000.0 | ml | - | - | |
1 Adjust the pH to 7.3 with NaOH 4M | |||||
2 Add to 1000 ml of medium after autoclaving and cooling to 55°C: | |||||
Trace element solution SL-10 | 1.0 | ml | - | - | |
Vitamin solution | 1.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 27.20 | g | 27.2 | 465.435 |
MgCl2 x 6 H2O | 9.06 | g | 9.06 | 44.564 |
CaCl2 x 2 H2O | 2.60 | g | 2.6 | 17.685 |
KCl | 1.28 | g | 1.28 | 17.17 |
MgSO4 x 7 H2O | 11.88 | g | 11.88 | 48.199 |
Na2HPO4 x 2 H2O | 0.02 | g | 0.02 | 0.141 |
NH4NO3 | 4.20 | mg | 0.004 | - |
Double distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
D-Calcium pantothenate | 5.0 | mg | 0.005 | - |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.3 |
Edit in Medium builder |
All strains for this medium: | 1 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 27.2 | 465.435 |
Agar | 20 | 59.465 |
Trypticase peptone | 17 | - |
MgSO4 x 7 H2O | 11.88 | 48.199 |
MgCl2 x 6 H2O | 9.06 | 44.564 |
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