1691 .
Artificial sea water - Tryptone soy broth Medium (ASW TSB Medium)
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Selected strain: Rhodococcus marinonascens DSM 43752
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D-Glucose | 2.5 | g | ||
|
|
Agar
(Bacto) |
20.0 | g | ||
|
|
Artificial Sea Water Solution 2x concentrated (ASW)
(or Biomaris) |
1000.0 | ml | ||
| 1 Adjust the pH to 7.3 with NaOH 4M | |||||
| 2 Add to 1000 ml of medium after autoclaving and cooling to 55°C: | |||||
|
|
Trace element solution SL-10 | 1.0 | ml | ||
|
|
Vitamin solution | 1.0 | ml | ||
Artificial Sea Water Solution 2x concentrated (ASW) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 27.20 | g |
|
|
MgCl2 x 6 H2O | 9.06 | g |
|
|
CaCl2 x 2 H2O | 2.60 | g |
|
|
KCl | 1.28 | g |
|
|
MgSO4 x 7 H2O | 11.88 | g |
|
|
Na2HPO4 x 2 H2O | 0.02 | g |
|
|
NH4NO3 | 4.20 | mg |
|
|
Double distilled water | 1000.00 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
D-Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |