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DSMZ

DSMZ 1697 . THIOSOCIUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1697 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 16.00 g 15.968 273.239
Na2SO4 2.50 g 2.495 17.566
CaCl2 x 2 H2O 1.00 g 0.998 6.788
KCl 0.45 g 0.449 6.024
NH4Cl 0.25 g 0.25 4.664
K2HPO4 0.14 g 0.14 0.802
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
HEPES buffer
(1 M, pH 8.0) 		20.00 ml 4.766 20
MgCl2 x 6 H2O 6.00 g 5.988 29.454
NaHCO3 1.00 g 0.998 11.88
Na2S2O3 x 5 H2O 2.50 g 2.495 10.053
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 980.00 ml - -
1 Dissolve ingredients (except HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins), dispense medium in test tubes or Erlenmeyrer flasks to 20% of volume, close with screw caps and autoclave. Add HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 8.0.

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 21.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 15.968 273.239
MgCl2 x 6 H2O 5.988 29.454
HEPES buffer 4.766 20
Na2SO4 2.495 17.566
Na2S2O3 x 5 H2O 2.495 10.053
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain