DSMZ 1697 . THIOSOCIUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 16.00 g 16 273.785
Na2SO4 2.50 g 2.5 17.601
CaCl2 x 2 H2O 1.00 g 1 6.802
KCl 0.45 g 0.45 6.036
NH4Cl 0.25 g 0.25 4.674
K2HPO4 0.14 g 0.14 0.804
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
HEPES buffer
(1 M, pH 8.0)
20.00 ml 4.766 20
MgCl2 x 6 H2O 6.00 g 6 29.513
NaHCO3 1.00 g 1 11.904
Na2S2O3 x 5 H2O 2.50 g 2.5 10.073
Wolin's vitamin solution 10.00 ml - -
Distilled water 970.00 ml - -
1 Dissolve ingredients (except HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins), dispense medium in test tubes or Erlenmeyrer flasks to 20% of volume, close with screw caps and autoclave. Add HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 8.0.
Solution occurs in 27 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 16 273.785
MgCl2 x 6 H2O 6 29.513
HEPES buffer 4.766 20
Na2SO4 2.5 17.601
Na2S2O3 x 5 H2O 2.5 10.073
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain