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DSMZ

DSMZ 1699 . SULFURIMONAS CRATERIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1699 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 981 ml - -
Solution B 25 ml - -
Solution C 1 ml - -
1 Dissolve compounds of solution A except sulfur and sparge it with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials that contain the appropriate amount of sulfur and autoclave at 110°C for 20 min. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.5 - 8.0, if necessary.

Solution A #

981
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.204 1.498
NH4Cl 0.25 g 0.255 4.764
NaCl 10.00 g 10.194 174.43
MgCl2 x 6 H2O 0.40 g 0.408 2.006
KCl 0.50 g 0.51 6.837
CaCl2 x 2 H2O 0.15 g 0.153 1.04
NaNO3 0.85 g 0.866 10.194
Modified Wolin's mineral solution 1.00 ml - -
Sulfur
(powdered) 		5.00 g 5.097 158.929
Distilled water 980.00 ml - -

Solution B #

25
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.25 g 50 471.752
Distilled water 25.00 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 24.07.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5 - 8.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 4.965 154.826
Na2CO3 1.241 11.712
NaNO3 0.844 9.931
KCl 0.497 6.66
MgCl2 x 6 H2O 0.397 1.954
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain