1700
1700 .
TRICHLOROBACTER AMMONIFICANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1700 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.80 | g | 0.798 | 5.867 | |
| K2HPO4 | 0.80 | g | 0.798 | 4.584 | |
| KNO3 | 0.50 | g | 0.499 | 4.936 | |
| Na-acetate | 0.80 | g | 0.798 | 9.733 | |
|
L-Methionine
(1% w/v) |
0.80 | ml | 0.008 | 0.054 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Na-thioglycolate | 0.10 | g | 0.1 | 0.875 | |
| Ascorbic acid | 0.10 | g | 0.1 | 0.567 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
| MgSO4 x 7 H2O | 0.15 | g | 0.15 | 0.607 | |
| Na2CO3 | 1.00 | g | 0.998 | 9.416 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve all ingredients except thioglycolate, ascorbic acid, calcium chloride, magnesium sulfate and carbonate, adjust pH to 6.7 and then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve thioglycolate and ascorbic acid, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride and magnesium sulfate from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Adjust pH of complete medium to pH 7.0 - 7.2, if necessary. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 17.07.23 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |